Multiplex Nested PCR for Vibrio Cholera v1

Primers targeting various regions of the V. Cholera genome were obtained from the literature or designed, and optimized for a multiplex nested PCR assay. The following primers were extracted from Hoshino et al., 1998: O1_rfbFor_Inner (modified here by Dr Alex Shaw), O1_rfbRev_Inner, O139_rfbFor_Inner, O139_rfbRev_Outer. The following primers were obtained from Theron et al., 2000: ctxA Inner Rev, ctxA_rev. The following primers were obtained from Nandi et al., 2000: ctxA_For, ompW_For, ompW_Rev, ompW_Inner_Rev. The following primers were designed by Dr Alex Shaw and are unpublished: ToxR_For_Outer, Tox_Rev_Outer, ToxR_For_Inner, O1_rfbFor_Outer, O1_rfbRev_Outer, O139_rfbFor_Outer, O139_rfbRev_Outer.