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Considering the number of available methods in molecular and cellular biology and the rapid development of new technologies, the need for an updated guide on what is currently applied and how to choose the best method for a specific goal has increased. Gold standard methods are known for their accuracy and reliable data under certain circumstances, allowing replicability. This perspective aims to characterize and define the current gold standard techniques from the last five years, enlighten their historical background, cite drawbacks and benefits, and delimit possible future aspects.
Kittelson et al. (Wed,) studied this question.