Screening of antibacterial producing endophytic bacteria isolated from Medicinal plant Coleus amboinicus

The objective of the current study was isolation of endophytic bacteria from Coleus amboinicus L. leaves and to evaluate its metabolite efficacy. Totally 5 bacterial colonies were isolated by direct plating method of which three strains are Gram positive in nature. Among 5, three were oxidase positive and all are catalase positive in nature. Isolate strain (CA2) is endospore producing rod bacterium identified as Bacillus sp showing positive antibacterial effect on test pathogens. The antimicrobial potential of endophytes reveals that isolate Bacillus sp has potent promising antibacterial agent production against Escherichia coli, Proteus vulgaris, K.pneumoniae and Candida albicans. The strain antibiotic production is largely regulated by dextrose, moderately by fructose and lactose and not by sucrose and sodium acetate. The compound is isolated and metabolite profile is identified by GCMS revealing 45 peak and 40 different compounds with important pharmacologically known active molecules. Strychane, 1-acetyl-20.alpha.-hydroxy-16-methylene, photocitral B, 2-2-(Benzoyloxy)ethoxyethyl benzoate,3-nonanone, guanethidine and acebutolol were used for antimycobactrial drug screening by in silico against 3-Oxoacyl-acyl-Carrier protein Reductase. Molecular docking reveals that acebutolol and guanethidine are strong antimycobaterial agents. In conclusion, this study demonstrated that medicinal plants had a wide variety of cultivable endophytic bacteria and serve as a promising source for the production of industrially important organic acids.