What type of study is this?

This is a In Vitro Study study.

August 18, 2025Open Access

Figure 4 from PSA Secretion from Single Circulating Tumor Cells of Metastatic Castration-Naïve Prostate Cancer Patients

Key Points

CTC analysis shows significant differences in PSMA and CD45 expression, highlighting tumor cell characteristics.
Nanowell chips were utilized to seed sorted DLA samples from patients with metastatic castration-naïve prostate cancer.
Observational analysis using a nanowell chip demonstrated the detection of cancer cell markers in patient samples which may aid diagnosis.
Findings suggest that high PSMA levels correspond with active tumor presence, guiding potential treatment interventions.

Abstract

Single-cell seeding of CTCs in a nanowell chip. Images of nanowells after seeding of sorted DLA samples. Nanowells 1 and 2 show calcein+ PSMA+ CD45− cells and nanowells 3 and 4 show calcein+ PSMA− CD45− cells. The green bar provides an estimate of the intensity levels per image. The cells that are stained brightly are viewed as positive. The red dashed line is set at the edge of the green bar of thumbnails 3 and 4. PSMA intensity (green bar) beyond this threshold indicates a positive signal as seen in the nanowells 1 and 2. CD45 intensity beyond this threshold indicates a positive signal and the presence of leukocytes in nanowells 5 and 6.

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