Sources of mismeasurement of RNA knockdown by DNAzymes and XNAzymes

Key Points

• RNA knockdown is significantly impacted by the mismeasurement of catalysts, with implications for therapy.
• Investigators found that DNAzymes and XNAzymes exhibit mismeasurement issues that can affect their efficacy in vivo.
• Approach includes a comprehensive analysis of RNA-cleaving catalysts to evaluate their knockdown efficiency and potential errors.
• Understanding mismeasurement aids in refining therapeutic techniques, highlighting the need for careful assessment in RNA-targeted therapies.