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This is a Cross-Sectional Study study.

September 12, 2025Open Access

First report of cereal yellow dwarf virus (CYDV-RPS) on maize in Bosnia and Herzegovina

Key Points

CYDV-RPS was detected in a maize sample, highlighting its potential impact on cereal crops.
Out of 60 collected samples, only one maize sample tested positive for CYDV using RT-PCR assays.
The study utilized ELISA and RT-PCR methods to assess virus presence in diverse crop samples.
The findings emphasize the need for monitoring CYDV as it can threaten maize production in Bosnia.

Abstract

A survey was conducted in Bosnia and Herzegovina in 2023, to investigate the presence of several important viruses affecting cereals, particularly those associated to cereal yellow dwarf viruses (CYDVs) and barley yellow dwarf viruses (BYDVs). Sixty leaf samples were collected, including 47 from maize plants (Zea mays L.) and 13 from barley plants (Hordeum vulgare L.), from across four grain-producing regions (Odzak, Sarajevo, Gornji Vakuf and Ilidza). Assessments for both groups of viruses, using ELISA and RT-PCR assays, detected CYDV in one maize sample (hybrid BC 418B) out of the 60 samples assessed. Nucleotide sequence analysis of the RT-PCR amplicon (2476 bp) of Bosnian isolate from maize hybrid BC418B (GenBank no. PV476203) showed that the isolate had 99.1% similarity with the CYDV RPS Mexican isolate (RPV-Mex-1; GenBank no. NC002198). This is the first report of the presence of CYDV-RPS in Bosnia and Herzegovina.

First report of cereal yellow dwarf virus (CYDV-RPS) on maize in Bosnia and Herzegovina

Key Points

Abstract

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