Milk phospholipids for correcting bile acid composition in rats with experimental fatty hepatosis

The unique functions of the liver require detailed study, since hepatopathology is a serious veterinary problem, which also negatively affects the productivity of farm animals. It is possible to clarify the pathogenetic mechanisms and its development using artificial modeling of hepatopathology and determine the therapeutic efficacy of hepatoprotective drugs, especially based on raw materials of animal origin. The aim of the study was to determine the effect of the use of milk phospholipids in the composition of the bioadditive “FLP-MD” on the secretion of bile acids by the liver in artificial modeling of fatty hepatosis in rats. To reproduce hepatopathology, laboratory rats were administered intragastrically with a 4% solution of tetracycline hydrochloride at a dose of 0.25 g/kg of body weight for seven days and the bioadditive was used at a dose of 13.5 mg/kg of body weight for nine days. Bile samples were collected from rats by conducting acute experiments. Six fractions of conjugated bile acids were determined in bile samples by thin-layer chromatography. It was found that in laboratory rats with experimental fatty hepatosis, the processes of biotransformation of primary and secondary cholates by conjugation with taurine were inhibited. In particular, a decrease in the concentration of taurocholic acid in the bile of sick animals by 20.5%-38.1% (P < 0.01), and of the complex of taurochenodeoxycholic and taurodeoxycholic acids by 21.8%-25.7% (P < 0.05) was recorded. In the case of using the bioadditive “FLP-MD” in rats with experimental fatty hepatosis, the concentration of taurocholic, taurochenodeoxycholic and taurodeoxycholic acids in bile significantly increased. The concentration of glycoconjugated bile acids and free cholates corresponded to their level in the control. The use of the bioadditive “FLP-MD” based on milk phospholipids in experimental fatty hepatosis eliminated the negative impact of the antibiotic in a toxic dose on the processes of biotransformation and the formation of cholates. This allows to recommend the bioadditive “FLP-MD” based on milk phospholipids as a hepatoprotective agent in the case of the use of antimicrobial drugs in animals