Shotgun Metagenomic Sequencing of Bacterial Enrichments on the Oxford Nanopore Platform Using the R10 flow cell and Ligation sequencing Kit v2

Key Points

• Multiplexed amplicon libraries were successfully created, enhancing sequencing efficiency and versatility.
• Using ONT V14 chemistry with the R10 flow cell improved the quality and accuracy of the sequencing process.
• The protocol streamlines the DNA extraction from various bacterial enrichments, enabling broader applications in microbial research.
• This technique opens avenues for studying complex bacterial communities across different environments.