Shotgun Metagenomic Sequencing of Bacterial Enrichments on the Oxford Nanopore Platform Using the R10 flow cell and Ligation sequencing Kit v2

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This is a Experimental Study study.

Christopher Duda; Padmini Ramachandran

doi:10.17504/protocols.io.81wgbkjqqgpk/v2

June 16, 2025

Shotgun Metagenomic Sequencing of Bacterial Enrichments on the Oxford Nanopore Platform Using the R10 flow cell and Ligation sequencing Kit v2

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Key Points

Multiplexed amplicon libraries were successfully created, enhancing sequencing efficiency and versatility.
Using ONT V14 chemistry with the R10 flow cell improved the quality and accuracy of the sequencing process.
The protocol streamlines the DNA extraction from various bacterial enrichments, enabling broader applications in microbial research.
This technique opens avenues for studying complex bacterial communities across different environments.

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Implication

This protocol produces multiplexed amplicon libraries from DNA extracted from bacterial enrichments, suggesting effective sequencing methods.

Abstract

This protocol produces multiplexed amplicon libraries from DNA extracted from general and selective bacterial enrichments using Nanopore's Ligation sequencing kit, suitable for sequencing on Oxford Nanopore Technologies‱ (ONT) MinION/Gridion systems using ONT V14 chemistry (SQK-NBD114-96).

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Duda et al. (2025) studied this question.

synapsesocial.com/papers/68d6c671b1249cec298b21ea https://doi.org/10.17504/protocols.io.81wgbkjqqgpk/v2

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Shotgun Metagenomic Sequencing of Bacterial Enrichments on the Oxford Nanopore Platform Using the R10 flow cell and Ligation sequencing Kit v2

Key Points

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

Shotgun Metagenomic Sequencing of Bacterial Enrichments on the Oxford Nanopore Platform Using the R10 flow cell and Ligation sequencing Kit v2

Key Points

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

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