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This is a Quantitative Study study.

October 27, 2025Open Access

Stimulation of Abscisic Acid Biosynthesis by Ethylene with Suppressive Action of MiR396 in Postharvest Strawberry Fruits

RCRenchi ChenZheJiang Academy of Agricultural Sciences YYYan YZheJiang Academy of Agricultural Sciences WGWeiliang GuanGuangxi University

Key Points

Ethylene enhances abscisic acid levels, driving the ripening of strawberry fruits and indicating active metabolic signaling.
The study found a 1.30-fold increase in ABA levels due to ethylene, with miR396 acting as a suppressor of ABA biosynthesis.
Crosstalk between ethylene and ABA was assessed using dual-luciferase assays, revealing 0.56-fold suppression of FaNCED2 promoter activity by miR396.
Understanding this regulation provides insights into fruit ripening, with potential implications for postharvest handling of strawberries.

Abstract

Both abscisic acid (ABA) and ethylene play essential roles in the ripening process of strawberry fruit (Fragaria × ananassa Duch.). In this study, the crosstalk between ABA and ethylene involved in strawberry fruit ripening was investigated. The results showed that ethylene triggered a 1.30-fold increase in ABA levels by suppressing the expression of miR396. Meanwhile, the silencing of miR396 (STTM396 reporter) demonstrated that miR396 acts as a negative regulatory factor of ABA biosynthesis by repressing FaNCED2 expression. Additionally, dual-luciferase assays indicated a 0.56-fold suppression of FaNCED2 promoter activity by miR396. These findings reveal a novel regulatory mechanism in which ethylene promotes ABA biosynthesis through the suppression of miR396 expression in postharvest strawberry fruit.

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