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The deoxyribonucleic acid of bacteriophages T2, T4, and T6 contains glucose linked to the hydroxymethyl groups of hydroxymethylcytosine.The work of Sinsheimer, Volkin, Jesaitis, and of Lichtenstein and Cohen (l-4) established the presence of glucosyl-hydroxymethylcytosine residues in the deoxyribonucleic acid and indicated that there is a characteristic glucose to hydroxymethylcytosine ratio for each phage.Lehman and Pratt ( 5) have now published a detailed analysis showing four types of hydroxymethylcytosine residues which have the following distribution in these phages : Unglucosylated.in T2 25%, in T4 0, in T6 25% cr-Glucosyl.in T2 70'%, in T4 70%, in TG 3% P-Glucosyl.. in T2 0, in T4 30%, in TG 0 &Glucosyl-or-glucosyl (diglucosyl) in T2 5y0, in T4 0, in T6 72%
Kornberg et al. (Mon,) studied this question.