What question did this study set out to answer?

To establish a method for the early diagnosis of Plasmopara viticola in grapevines using quantitative PCR.

January 1, 2025Прикладная биохимия и микробиология / Applied Biochemistry and Microbiology0 citations

Identification of the Causal Agent of Downy Mildew of Plasmopara viticola Grapes by Quantitative PCR

Key Points

To establish a method for the early diagnosis of Plasmopara viticola in grapevines using quantitative PCR.
Utilized quantitative real-time PCR with SYBR Green I for detection.
Developed six pairs of primers specific for Plasmopara viticola.
Evaluated the effectiveness of primers using metagenomic data.
Achieved early detection with high accuracy using the designed primers.
Found a strong positive correlation with metagenomic data (R2 = 0.86).
Provided a basis for predicting and controlling downy mildew epidemics in vineyards.

Abstract

A new method is proposed for the early diagnosis of the causal agent of grapes downy mildew, Plasmopara viticola, based on the method of quantitative real-time PCR (qPCR RT) using SYBR Green I fluorescence. Six pairs primers were developed for the diagnosis of P. viticola, among the designed primers, PvITS1₂-real-s/a demonstrated the highest effectiveness for early detection of grapevine downy mildew with a strong positive correlation with the metagenomic data of P. viticola distribution in Far Eastern grape species and varieties, where a linear dependence was found (R2 = 0. 86). Thus, qPCR RT of PvITS1₂ can be used for early detection and monitoring of asymptomatic P. viticola infections. The developed method can be used as a basis for predicting epidemics of downy mildew of grapes and for its control in vineyards.

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