Investigation on Antibacterial and Wound Healing Properties of Zinc Phthalocyanine Derivatives Encapsulated in Poly(Lactic‐Co‐Glycolic Acid) Nanophotosensitizer

ABSTRACT Antibiotic abuse has given rise to bacterial resistance, which severely challenges the medical and public health domains. Distinct from traditional antibiotic sterilization methods, photodynamic sterilization has attracted significant attention due to its advantages of repeatable application, broad sterilization range, and multiple action targets. Therefore, zinc phthalocyanine 4‐phenoxyacetate encapsulated in poly(lactic‐co‐glycolic acid) (PLGA@ZPCC NPs) is synthesized in this study, and explored their bactericidal efficacy via photodynamic therapy (PDT). The physicochemical properties and singlet oxygen generation capacity of PLGA@ZPCC NPs are analyzed via NMR, EA, TEM, DLS, FTIR, and ESR. Photodynamic antibacterial experiments demonstrated that the PLGA@ZPCC NPsr exhibited favorable bactericidal effects and satisfactory biocompatibility. In a mouse skin wound infection model, PLGA@ZPCC NPs showed a notable advantage in promoting wound healing. Specifically, the healing outcomes of the medium and high‐dose (100, 250 mg/kg) of PLGA@ZPCC NPs are superior to the mupirocin (200 mg/kg). During the monitoring period, the mice showed no abnormal signs, such as impaired immune function or reduced body weight. Hematological routine tests and pathological examination indicated that PLGA@ZPCC NPs caused no significant damage or pathological alterations to the mice's major organ tissues. PLGA@ZPCC NPs exhibited excellent antibacterial activity, wound healing promotion, and biosafety.