The use of ZnO nanoparticles and biological products are considered cost-effective and environmentally friendly ways to increase crop yields. This work is devoted to the study of the effect of the combined use of a biological product LPB (liquid-phase biological product) and ZnO nanoparticles, synthesized using green tea extract and a ZnSO4∙7H2O solution with a concentration of 0.5 mol/l, on the germination energy, germination and biometry of fiber flax seedlings of the Tverskoy variety. The results of microbiological analysis of the biological product LPB-Zn showed that when ZnO nanoparticles were introduced into LPB, the number of ammonifying and amylolytic microorganisms decreased by 4.5 and 13.7 times compared to ZnO nanoparticles. The work studied two methods of using biological products: watering and inoculation. Thus, when irrigated with LPB and LPB-Zn, the germination energy was 76-89.5 and 77-87%, respectively, while germination in both options varied from 82 to 90.5%. The most pronounced positive effect of the combined use of LPB and ZnO nanoparticles for irrigation was noted when studying the average length of one seedling: in the LPB-Zn variant, a maximum average length of 14-15 cm was obtained, while when using LPB and ZnO nanoparticles for irrigation, the average length of one seedling was 12-14 cm. Inoculation of seeds in the biological preparation LPB-Zn, on the contrary, had a greater effect on the germination energy and germination of seeds, which increased compared not only to the control, but also to the variant LPB. At the same time, inoculation of seeds in the studied preparations did not affect either the average length or the average weight of one seedling. Thus, the combined use of the biological product LPB and ZnO nanoparticles is very promising. However, it requires additional research to determine the maximum concentration of ZnO nanoparticles in LPB at which its beneficial properties are lost.
Lyubimova et al. (Sat,) studied this question.