What question did this study set out to answer?

To evaluate the effects of amnion transplantation and collagen crosslinking on corneal healing following alkali burn in rabbits.

March 1, 2026

Effect of Amnion Membrane Transplantation and Crosslinking Treatment in a Rabbit Model of Corneal Alkali Burn

Key Points

To evaluate the effects of amnion transplantation and collagen crosslinking on corneal healing following alkali burn in rabbits.
Induced alkali injury on rabbit corneas using 1N NaOH.
Divided 32 rabbits into five treatment groups: control, alkali burned, amnion transplantation, crosslinking, and combined treatment.
Conducted H&E staining and TUNEL assay to assess histomorphology and apoptosis.
Evaluated inflammation using CD68 immunostaining.
Administered treatments over a duration of 21 days.
Group 4 (crosslinking) showed better epithelial regeneration compared to Group 2 (alkali burned, p=0.010).
Significant reduction in TUNEL(+) cells in the corneal epithelium and stroma in Group 4 compared to Group 2 (p=0.044).
Significant decrease in CD68(+) cell number in Groups 4 (p=0.001) and 5 (p=0.039) compared to Group 2.
Crosslinking treatment reduced corneal damage and inflammatory cell infiltration.

Abstract

Effects of Amnion Membrane Transplantation and Cross-linking Treatmentin a Rabbit Model of Corneal Alkali Burn Authors: Sevgi ÖZEL SUBAŞI (1), Melda YARDIMOGLU (2), Yusufhan YAZIR (3), Selenay FURATRENÇBER (2), Kübra KAVRAM (4), Özgül ALTINTAŞ (1), Süleyman KARAMAN (1)1. Department of Ophthalmology, Faculty of Medicine, Kocaeli University, Kocaeli, TURKEY2. Department of Histology and Embryology, Faculty of Medicine, Kocaeli University, Kocaeli, TURKEY3. Department of Histology and Embryology; Center of Stem Cell and Gene Therapies Research and Practice,Faculty of Medicine, Kocaeli University, Kocaeli, TURKEY4. Department of Histology and Embryology, The Institute of Health Sciences, Kocaeli University , Kocaeli,TURKEYDOI: 10.1002/EMC2016.0004Corresponding email: melda.yardimoglu@gmail.comKeywords: alkali burn, cross-linking treatment, amnion transplantation Eye burn is widely seen and may be caused by various chemical and physical agents including acids, alkalis, fire, etc. They are most commonly a consequence of chemical handling accidents and may result in permanent damage to the ocular surface and visual function(1-4). We aimed to evaluate the effect of amnion transplantation and collagen crosslinking treatments after alkali burn in rabbit corneas. 32 New Zealand white rabbits were divided into five groups as control (group 1? n=5), alkali burned (group 2?n=8), amnion transplantation treatment (group 3? n=8), crosslinking treatment (group 4? n=8), and combined treatment (group 5? n=8). Alkali injury was induced only in the right corneas of rabbits using 1N NaOH except the control group. After the alkali burn, group 3 was applied amnion transplantation, group 4 was applied collagen crosslinking, and group 5 received combined treatment. All groups were performed medical treatmentduring 21 days. Histomorphology was evaulated with H4:994–1002.

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