A Dual-Mode Aptasensor Integrating Ratiometric Fluorescence and Colorimetry for On-Site Cu2+ Detection

Key Points

• This research aims to develop a dual-mode aptasensor for the rapid and selective detection of Cu2+.
• Development of the RaCApt dual-mode aptasensor
• Integration of ratiometric fluorescence and colorimetry
• Utilization of aptamer conformational switching for detection
• Achieves detection of Cu2+ in less than 5 seconds
• Demonstrates high selectivity for Cu2+ over other ions
• Provides dual outputs via fluorescence and colorimetric signals