What question did this study set out to answer?

The aim is to compare the performance of three CUT&RUN kits for profiling histone marks and their impact on data quality.

March 21, 2026Open Access

A side-by-side comparison of commercial CUT&RUN kits

The aim is to compare the performance of three CUT&RUN kits for profiling histone marks and their impact on data quality.
Evaluated three commercial CUT&RUN kits on specific histone marks.
Compared data quality from libraries generated with fresh and frozen cells.
Analyzed libraries created with varying cell input numbers.
All three kits produced high-quality libraries.
CST protocol showed superior signal and accuracy despite fewer detected peaks for H3K4me3.
EpiCypher achieved the highest accuracy for H3K27me3 libraries.

CUT libraries generated with 100,000 cells had higher concordance between technical replicates than did libraries made with 500,000 cells.

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