Abstract 7673: 9-channel spatial co-detection of clinically relevant protein and RNA targets in FFPE tumors with HCR™ Gold and MoxiePlex. | Synapse
April 5, 2026
Abstract 7673: 9-channel spatial co-detection of clinically relevant protein and RNA targets in FFPE tumors with HCR™ Gold and MoxiePlex.
Key Points
The aim is to assess a new method for simultaneous detection of protein and RNA in fixed tumor samples, overcoming limitations of existing techniques.
Used HCR™ Gold IF for protein detection and HCR™ Gold RNA-FISH for RNA detection.
Tested 7-plex and 9-plex configurations on the MoxiePlex imaging platform.
Generated single-stain reference slides for exposure and spectral bleed-through settings.
Performed whole-slide imaging in a single acquisition round with automated spectral unmixing.
Achieved bright signals while preserving tissue architecture.
Localized protein and RNA targets correctly within tumor and stroma regions.
Demonstrated consistent imaging performance suitable for routine multiplex scanning.
Abstract
Abstract Background: High-plex spatial profiling of protein Part 1 (Regular Abstracts); 2026 Apr 17-22; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2026;86(7 Suppl):Abstract nr 7673.