Abstract 4157: Spatial immune profiling of solid tumors by complementing H&E with a novel 17-plex immunofluorescence approach

Key Points

• To enhance the identification of cellular neighborhoods in solid tumors by integrating multiplex immunofluorescence with H&E staining.
• Validated a 17-plex immunofluorescence assay on the Orion platform.
• Aligned same-slide H&E images with fIHC to assess cellular neighborhoods.
• Employed HALO software for cell phenotyping and slide registration.
• Supported identification of regions with tumor-infiltrating lymphocytes and immune deserts.
• Improved interpretability of spatial patterns by linking cell phenotypes with tissue morphology.
• Demonstrated adaptability of the workflow across various tumor types.

Abstract