Abstract 3027: Cellular uptake of nanoliposomes with optimized cholesterol concentration and lipid extract derived from a model low grade serous ovarian carcinoma cell line

Abstract Introduction: Low-grade serous ovarian carcinoma (LGSOC) represents a rare subtype of epithelial ovarian cancer, accounting for less than 10% of all cases and typically diagnosed in younger women. The disease is characterized by tumor growth driven by activating mutations in the MAPK pathway, including KRAS, BRAF, and NRAS. Despite its slow progression, LGSOC remains challenging to treat due to limited responsiveness to conventional chemotherapy. The objective of this study was to isolate cellular lipid extract (LE) material from a model LGSOC cell line, and to utilize the newly acquired LE material to develop a relatively target specific nanoliposomal system. Early formulation and in vitro studies include optimization for cholesterol and LE content. Methods: Low-grade serous ovarian carcinoma cell line (PM-LGSOC-01, Cytion) was cultured and expanded in EMEM growth medium supplemented with 10% FBS. The PM-LGSOC-01-lipid extract (LE) material was extracted from PM-LGSOC-01 when the cells reached ∼90% confluency. Lipid extraction was performed as described previously (Alharbi Part 1 (Regular Abstracts); 2026 Apr 17-22; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2026;86(7 Suppl):Abstract nr 3027.