Nanoliposome Uptake Optimization in Low Grade Serous Ovarian Carcinoma Cells

Introduction: Low-grade serous ovarian carcinoma (LGSOC) represents a rare subtype of epithelial ovarian cancer, accounting for less than 10% of all cases and typically diagnosed in younger women. The disease is characterized by tumor growth driven by activating mutations in the MAPK pathway, including KRAS, BRAF, and NRAS. Despite its slow progression, LGSOC remains challenging to treat due to limited responsiveness to conventional chemotherapy. The objective of this study was to isolate cellular lipid extract (LE) material from a model LGSOC cell line, and to utilize the newly acquired LE material to develop a relatively target specific nanoliposomal system. Early formulation and in vitro studies include optimization for cholesterol and LE content. Methods: Low-grade serous ovarian carcinoma cell line (PM-LGSOC-01, Cytion) was cultured and expanded in EMEM growth medium supplemented with 10% FBS. The PM-LGSOC-01-lipid extract (LE) material was extracted from PM-LGSOC-01 when the cells reached ∼90% confluency. Lipid extraction was performed as described previously (Alharbi & Campbell, AAPS Open, number: 5(2018)). Nanoliposomal preparations consisted of DOPC, cholesterol, and LE at various ratios and wereformed by thin film method using a Buchi R-80 rotary evaporator. DPPE-Rhodamine was included in the preparations for cellular studies. Following sonication, particle size and zeta potential values weredetermined using ZetaPals. Fluorescence detection was performed using a fluorescence microplate reader. Results: Three preparations (1) DOPC (100%), (2) DOPC/Chol (95/5), and (3) DOPC/Chol (90/10) wereprepared with an average particle size of 221 ± 3 nm, 250 ± 5 nm, and 139 ± 0.3 nm, respectively. Preliminary results suggest that the additional inclusion of cholesterol in the nanoliposomal preparations increased their uptake by the target (PM-LGSOC-01) cells. Studies investigating the influence of PM-LGSOC-01-LE on the uptake of nanoliposomes by the target cells are currently underway. Conclusion: To date, the influence of cholesterol on the uptake of nanoliposomes by LGSOC cells has been investigated. Future studies will evaluate the effect of optimized cholesterol and LE contenton targeting low-grade serous ovarian carcinoma. Citation Format: Sofia Orlando, Krishna Panchal, Heer Patel, Robert B. Campbell. Cellular uptake of nanoliposomes with optimized cholesterol concentration and lipid extract derived from a model low grade serous ovarian carcinoma cell line [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2026; Part 1 (Regular Abstracts); 2026 Apr 17-22; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2026;86(7 Suppl):Abstract nr 3027.