Differential modulation of gentamicin antibacterial activity by vitamin B12 and vitamin D3 against Gram-positive and Gram-negative bacteria: an in vitro–in silico study

Key Points

• The study aims to evaluate the effects of Vitamin B12 and Vitamin D3 on gentamicin's antibacterial activity against different bacterial strains.
• In vitro experiments were conducted to test the antibacterial effects of Vitamin B12 and Vitamin D3 in combination with gentamicin.
• Comparative analysis of efficacy was performed on both gram-positive and gram-negative bacteria.
• Vitamin B12 showed a dose-sparing effect on gentamicin's antibacterial activity.
• Vitamin D3 potentially compromised the efficacy of gentamicin under the tested conditions.