Evaluation of single-stranded DNA knock-in and base editing in combination with sleeping beauty transposon for a non-viral allogeneic 19BBz CAR-T generation

Key Points

• The study aims to evaluate the effectiveness of single-stranded DNA knock-in and base editing in generating CAR-T cells.
• Evaluated the combination of base editing and sleeping beauty transposon on CAR-T generation
• Utilized non-viral methods for allogeneic CAR-T development
• Demonstrated successful generation of 19BBz CAR-T cells using the evaluated techniques
• Showed enhanced transfection efficiency with the combined approach