Scale-up of plasmid linearization for mRNA vaccine production: from development to pilot scale

Key Points

• The research aims to scale up the linearization of plasmids for efficient mRNA vaccine production.
• Development of plasmid linearization techniques for mRNA production.
• Pilot-scale production process was established and tested.
• Collaboration with Bio-Manguinhos for scaling efforts.
• Successful linearization of plasmids for mRNA production was achieved.
• Pilot-scale methods displayed efficiency suitable for broader vaccine production.
• Results support the feasibility of scaling up mRNA vaccine manufacturing.