What does this research mean for the field?

Supplementation with specific optimal concentrations of green tea extract and (+)-catechin enhances ram sperm quality and reduces oxidative stress during chilled storage, whereas high concentrations of green tea extract are detrimental. Novelty: ClaimNovelty.INCREMENTAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

This research aims to evaluate the effects of green tea extract and (+)-catechin on sperm quality parameters during chilled storage in Arabi rams.

June 4, 2026Open Access

Effects of Green Tea Extract and (+)‐Catechin Hydrate on Sperm Quality and Seminal Plasma Antioxidant Activity: An In Vitro Study in Rams

Key Points

This research aims to evaluate the effects of green tea extract and (+)-catechin on sperm quality parameters during chilled storage in Arabi rams.
Pooled semen was supplemented with varying concentrations of GTE and (+)-catechin during in vitro storage.
Sperm quality parameters were assessed at 1, 24, 48, and 72 hours of storage.
The levels of MDA were measured to evaluate antioxidant activity at 72 hours.
GTE (50 and 100 µg/mL) and catechin (600 and 800 µg/mL) significantly improved sperm motility compared to control (p < 0.05).
Viability and membrane integrity were enhanced at lower GTE concentrations and all catechin concentrations (p < 0.05).
MDA levels were significantly lower in treatment groups, indicating better antioxidant activity at various concentrations.

Abstract

BACKGROUND: Antioxidants have gained significant attention for improving sperm parameters. Green tea and catechin, known for their potent antioxidant properties, are particularly interesting in this context. OBJECTIVE: This study investigated the in vitro effects of green tea extract (GTE) and (+)-catechin on semen quality parameters of Arabi rams during chilled storage. METHODS: The pooled and diluted semen was divided and supplemented with GTE and (+)-catechin (50, 100, 150 and 200 µg/mL levels). The control group did not receive any supplements. Sperm quality and semen pH were evaluated at 1, 24, 48 and 72 h after storage. Seminal plasma MDA level was measured at 72 h of semen storage. RESULTS: Supplementation with GTE and (+)-catechin improved sperm motility more than control (p < 0.05). Specifically, GTE at 50 and 100 µg/mL enhanced motility, while higher concentrations were detrimental. Catechin at 600 and 800 µg/mL maintained motility better than the control at 72 h. Sperm viability and membrane integrity were significantly improved at 50 and 100 µg/mL GTE and all catechin concentrations (p < 0.05) compared to the control. However, higher GTE concentrations (150-200 µg/mL) decreased sperm viability and membrane integrity at 72 h. Additionally, morphological abnormalities were reduced by 100 µg/mL GTE and the higher catechin concentrations in comparison to the control. Seminal plasma pH varied over the 72-h storage period, but no significant differences were observed between the experimental and control. MDA levels were significantly lower (p < 0.05) in the GTE (50-200 µg/mL) and catechin (200-800 µg/mL) treatment groups, compared to the control at 72 h. CONCLUSIONS: GTE and catechin enhance semen parameters during chilled storage, with optimal effects at specific concentrations. High levels of GTE may negatively impact sperm quality, underscoring the importance of precise dosing in semen preservation.

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