Here, we present a protocol for identifying human innate lymphoid cells (ILCs) and T cells using a 19-plex flow cytometry panel. We describe steps for titrating antibodies, preparing single-stain controls and samples, and staining cells for human peripheral blood and bone marrow samples. We then detail procedures for transferring a flow cytometry panel optimized for conventional cytometry to spectral flow cytometry analysis. This protocol facilitates correlation with murine models, maximizes measurable parameters, resolves complex fluorophore combinations, and increases panel flexibility.
Acklam et al. (Thu,) studied this question.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: