A hydrophilic interaction UPLC-MS/MS quantitative method for the quantification of saracatinib in the human liver microsome matrix and its application in in vitro metabolic stability assessment

Key Points

• The aim is to quantify saracatinib in human liver microsome matrices and assess metabolic stability.
• Employs a hydrophilic interaction UPLC-MS/MS technique.
• Assesses saracatinib levels in human liver microsomes.
• Investigates structural modifications to enhance metabolic stability.
• Successful quantification of saracatinib in the HLM matrix.
• Minor structural changes may lead to better metabolic stability in new derivatives.