Background: Acute exercise induces measurable inflammatory and hematological responses, which may differ according to training status. Methods: Trained soccer players and sedentary controls completed a progressive exercise test until exhaustion. Blood samples were collected at six time points (up to 48 h post-exercise). Gene expression was assessed using real-time PCR, protein concentration via ELISA, and leukocyte, lymphocyte and neutrophil counts through routine hematology. Analyses included between-group comparisons, within-group time effects, and gene–protein correlations. Results: Sedentary individuals showed delayed increases in TNF-α mRNA (p < 0.05), whereas athletes maintained stable levels. Athletes exhibited consistently higher TNFR-1 expression (p < 0.05), while TNFR-2 showed no differences between groups. IL-4 expression was higher in athletes 30 min (p = 0.038) and 24 h after test (p = 0.002), whereas IL-4 protein decreased in sedentary subjects after 6 h (p = 0.009) and 24 h (p = 0.019). IL-4Rα was transiently elevated post-exercise in sedentary individuals and briefly suppressed in athletes (p = 0.041). Hematological responses showed stronger leukocytosis and neutrophilia in sedentary individuals. Conclusions: The temporal inflammatory patterns differed substantially between groups, indicating more efficient resolution dynamics in trained individuals. These findings highlight practical value for monitoring adaptation and recovery.
Małkowska et al. (Tue,) studied this question.
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