One-pot synthesis of fluorescent cap1 mRNAs using labelled trinucleotide m7G-cap analogs for real-time in vitro and in vivo mRNA tracking

Key Points

• The aim is to develop a simple method for creating mRNAs that can be tracked in cells and organisms using fluorescent markers.
• One-pot synthesis of cap1 mRNAs using fluorophore-tagged trinucleotide analogs.
• Application of synthesized mRNAs for both in vitro and in vivo studies.
• Tracking of mRNA distribution in cellular imaging and animal models.
• Successfully synthesized trackable mRNAs using cap1 analogs.
• Fluorescent mRNAs showed effective tracking in cellular imaging.
• Demonstrated broad distribution of mRNAs in whole animal studies.