October 1, 1971Open Access
Effect of Pactamycin on Synthesis of Poliovirus Proteins: a Method for Genetic Mapping
Key Result
Pactamycin differentially reduced the synthesis of poliovirus proteins in infected HeLa cells, indicating their relative distance from the initiation site and suggesting a specific gene order.
Structured PICO
P
Population
poliovirus-infected HeLa cells
I
Intervention
pactamycin
O
Outcome
spectrum of poliovirus proteins synthesized (relative amounts of NCVP 1, NCVP X, and NCVP 2)surrogate
Pactamycin's differential inhibition of poliovirus protein synthesis provides a method for genetic mapping of the viral genome.
Abstract
We have studied the effect of the drug pactamycin on protein synthesis in poliovirus-infected HeLa cells. At a concentration which primarily inhibits initiation of protein synthesis, the spectrum of poliovirus proteins synthesized is markedly changed. The amount of NCVP 1, the capsid precursor, is greatly reduced relative to NCVP 2 and the amount of NCVP X is slightly reduced. Since it is believed that there is only one major site for the initiation of protein synthesis on the poliovirus genome, we interpret this differential effect on the poliovirus proteins to be an indication of their relative distance from the initiation site. On this basis, we propose a gene order for the poliovirus genome (5' --> 3') of NCVP 1, NCVP X, NCVP 2.