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Abstract Background The objective of the present study was to evaluate in vitro the cytotoxicity and bioactivity of various endodontic sealers (CeraSeal, BioRoot™ and AH Plus ® ) in pre-osteoblast mouse cells (MC3T3 cells). Methods MC3T3 cells (ATCC CRL-2594) were plated in 1 × 10 4 cells/well in 96-well plates in contact with endodontic sealers at concentrations of 1:10 and 1:100. Cell viability was evaluated by MTT assay after 24 and 48 h. In addition, sealer bioactivity was measured by RT-PCR for mediator of inflammation ( Tnf, Ptgs2 ) and mineralization ( Runx2, Msx1, Ssp1 and Dmp1 ) after 24 h and by Alizarin Red S Assay of mineralization after 28 days. Data were analyzed using one-way ANOVA followed by the Tukey’s post-test at a significance level of 5%. Results BioRoot™ presented 24-hour cytotoxicity ( p 0.05). TNF-α gene expression was induced by AH Plus ® ( p 0.05). Conclusion CeraSeal, BioRoot™ and AH Plus ® sealers were not cytotoxic to MC3T3 cells within 48 h, but differentially induced the expression of genes related to inflammation and mineralization without impacting biomineralization by the cells.
Almeida‐Junior et al. (Thu,) studied this question.