Functionalized Two-Dimensional Nanochannel Membranes to Distinguish Methylated/Unmethylated Peptides for Sensing Cellular G9a Protein

Key Points

• The aim is to create membranes that can differentiate between methylated and unmethylated peptides associated with the G9a protein.
• Developed functionalized two-dimensional nanochannel membranes.
• Conducted assessments of peptide binding and separation.
• Analyzed binding affinities and specificity for methylation status.
• Demonstrated successful differentiation of methylated and unmethylated peptides.
• Achieved high sensitivity in detecting changes in peptide profiles related to G9a activity.