What question did this study set out to answer?

This project aims to enhance the wellbeing of nude mice while ensuring more reliable experimental outcomes in oncology.

February 6, 20260 citationsOpen Access

Improving housing conditions in tumor xenograft mice for better refinement

Key Points

This project aims to enhance the wellbeing of nude mice while ensuring more reliable experimental outcomes in oncology.
Compared housing at thermoneutrality (30° C) to conventional housing (22° C) in nude mice.
Measured wellbeing, tumor growth, and treatment success through various imaging and analysis techniques.
Assessed biodistribution and efficacy of radioligand therapy.
Utilized PET/CT and autoradiography for imaging evaluations.
Tumor growth was improved in mice housed at thermoneutrality compared to conventional conditions.
Radioligand uptake was notably higher in thermoneutral housing.
Validated improvements may lead to refined housing practices in oncology and nuclear medicine.

Abstract

This animal housing refinement project aims to improve the wellbeing of nude mice used in experimental oncology and nuclear medicine, while increasing the robustness and reliability of studies performed with these animals. The project proposes that housing at thermoneutrality (30° C) versus conventional housing (22° C) improves tumor growth independent of Matrigel, radioligand imaging and efficacy of radioligand therapy. These hypotheses will be verified by measuring wellbeing, tumor growth, radioligand uptake (biodistribution, PET/CT, autoradiography) and treatment success (survival, growth inhibition). If validated, the results of this project may initiate a change towards refined housing practices for nude mice in experimental oncology and nuclear medicine.

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