Investigation of RyR1 allosteric modulation through the nucleotide binding pocket

Key Points

• The aim is to explore how changes in the nucleotide binding pocket of RyR1 affect its function.
• Experimental modulation of RyR1 through specific nucleotide interactions.
• Analysis of calcium release activity in skeletal muscle samples.
• Use of computational modeling to assess structural changes.
• Identified key nucleotide interactions that influence RyR1 activity.
• Demonstrated a significant increase in calcium release under specific modulation conditions.