ABSTRACT With the increasing prominence of population aging, antiaging research has garnered significant attention. This study investigated the antioxidant and antiaging effects of a crude Gynura divaricata polysaccharide (GDP) and its purified fractions (GDP1, GDP3), prepared via a H 2 O 2 /Vc‐assisted method. All GDPs exhibited potent radical‐scavenging activity in vitro. In a Caenorhabditis elegans model, GDPs significantly extended lifespan (up to 19.17%), enhanced motility, and increased heat stress resistance. Treatment with 2 mg/mL GDP markedly reduced lipofuscin accumulation by 43.6% and elevated superoxide dismutase (SOD) and catalase (CAT) activities. GDP demonstrated the strongest effects. Mechanistic studies revealed GDP upregulates daf‐16 , sod‐3 , and skn‐1 while downregulating daf‐2 and age‐1 . Crucially, the lifespan‐extending effect of GDP was abolished in daf‐16 mutants, demonstrating that their antiaging action operates primarily via the insulin/IGF signaling (IIS) pathway.
Liao et al. (Sun,) studied this question.