Non-specific Clones Hamper the Reliability of Ig Light Chain Gene Rearrangements in Next-Generation Sequencing-Based Measurable Residual Disease Monitoring in Pediatric B-Cell ALL: A Single-Center Cohort Study
Key Points
The study aims to evaluate how non-specific clones affect the reliability of IGK rearrangements in monitoring measurable residual disease (MRD) in pediatric B-cell acute lymphoblastic leukemia (B-ALL).
Analyzed IGK rearrangements in pediatric B-ALL using next-generation sequencing (NGS) for MRD monitoring.
Identified and filtered out non-specific clones to assess their impact on accuracy.
Conducted the study at a single center with a defined cohort of pediatric patients.
Non-specific clones were found to significantly reduce the reliability of IGK rearrangements.
Filtering these clones improved accuracy and reduced false positives.
The overall reliability of NGS-based MRD monitoring was enhanced with marked reductions in non-specific cloning effects.
Abstract
Non-specific clones reduce the reliability of IGK rearrangements for NGS-based MRD monitoring in pediatric B-ALL. Filtering out these clones may enhance accu-racy by reducing false positives.
Non-specific Clones Hamper the Reliability of Ig Light Chain Gene Rearrangements in Next-Generation Sequencing-Based Measurable Residual Disease Monitoring in Pediatric B-Cell ALL: A Single-Center Cohort Study | Synapse