What type of study is this?

This is a Validation Study study.

What question did this study set out to answer?

To optimize detection methods for polycyclic aromatic hydrocarbons in Barbus carpathicus tissues.

February 16, 2026Journal of Environmental Science and Health Part B0 citations

Optimization of detection of twelve polycyclic aromatic hydrocarbons in Carpathian barbel ( Barbus carpathicus ) tissues: extraction methods and GC-MS parameters

JLJana LakatošováSlovak University of Agriculture in Nitra MHMarek HelczmanSlovak University of Agriculture in Nitra MRMarcel RepiskýUniversity Hospital Bratislava

Key Points

To optimize detection methods for polycyclic aromatic hydrocarbons in Barbus carpathicus tissues.
Optimized GC-MS method in selected ion monitoring mode for twelve PAHs.
Evaluated HP-5ms and DB-EUPAH columns under various temperature programs.
Validated method for linearity, precision, detection, and quantification limits.
Achieved excellent linearity (R² ≥ 0.995) and low deviation from linearity (<20%).
Demonstrated superior results with shaker-assisted extraction and solid-phase extraction.
Recovery rates ranged from 63.1% to 146.0%, with eight PAHs meeting acceptable recovery criteria.

Abstract

A GC-MS method in selected ion monitoring (SIM) mode was optimized and validated for the determination of twelve polycyclic aromatic hydrocarbons (PAHs) in Barbus carpathicus tissues (muscle and hepatopancreas). Two chromatographic columns (HP-5ms and DB-EUPAH) were evaluated with multiple temperature programs, and the DB-EUPAH column proved superior for separating critical PAH isomers within a 25-minute runtime. Method validation demonstrated excellent linearity (R2 ≥ 0.995), with deviation from linearity B.carpathicus tissue analysis. The developed method provides a reliable, validated analytical tool suitable for routine monitoring and risk assessment of PAH contamination in B.carpathicus, supporting environmental monitoring and food safety protocols.

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