What question did this study set out to answer?

This research aims to explore dual centrifugation as a method for formulating RNA-loaded lipid nanoparticles (LNPs).

February 19, 2026RSC Pharmaceutics1 citationsOpen Access

Dual centrifugation – a novel perspective on lipid nanoparticle formulation development

VBV. BenderUniversity of Freiburg LFL. Yolanda FuchsUniversity of Freiburg MKMonika Köll-WeberUniversity of Freiburg

Key Points

This research aims to explore dual centrifugation as a method for formulating RNA-loaded lipid nanoparticles (LNPs).
Developed a novel dual centrifugation technique.
Optimized the process using design of experiments (DoE) for efficiency.
Scaled down to 1 mg batch sizes for practical use.
Demonstrated simplified preparation of RNA-loaded LNPs.
Achieved robust formulation development at small batch sizes.

Abstract

Dual centrifugation enables a simplified and cost-effective preparation of RNA-loaded LNPs. Downscaled and DoE optimized to 1 mg batch sizes, the method offers a robust platform for LNP formulation development.

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