Uncovering the Substrate Promiscuity of Mammalian PTGR2: Insights into Metabolic Crosstalk

Key Points

• This research aims to explore the metabolic function and substrate versatility of mammalian PTGR2, an ene-reductase enzyme.
• Utilized biochemical assays to analyze activity of PTGR2 with various substrates.
• Conducted comparative analysis with microbial ene-reductases to highlight differences.
• Employed molecular modeling to predict substrate binding and interaction mechanisms.
• Demonstrated that mammalian PTGR2 shows significant substrate promiscuity compared to microbial counterparts.
• Identified key substrates that enhance metabolic crosstalk in mammalian systems.
• Revealed potential implications for biocatalytic applications in metabolic engineering.