Sustainable Biomimetic Nanozymes: Catalase‐Like Nitrogen‐Doped Green Carbon Dots for Efficient Intracellular ROS Scavenging

ABSTRACT Oxidative stress induced by reactive oxygen species (ROS) plays a key role in various pathological conditions and is typically mitigated by natural antioxidants such as catalase. However, enzyme‐based therapies often suffer from limitations including high cost, instability across temperature and pH, and limited shelf life. To address these challenges, we report the green synthesis of nitrogen‐doped carbon dots (TNCDs) derived from the non‐edible plant Tradescantia pallida . These carbon dots exhibit catalase‐mimicking activity, effectively catalyzing the decomposition of H 2 O 2 and scavenging ROS both in vitro and within cells. TNCDs demonstrated strong antioxidant potential in ABTS and DPPH assays and showed significant intracellular ROS neutralization in MDA‐MB‐231 cells via DCFDA fluorescence analysis. The use of non‐edible biomass as a precursor not only ensures sustainability and cost‐effectiveness but also avoids competition with food resources. This study highlights TNCDs as a promising eco‐friendly nanozyme platform for oxidative stress‐related biomedical applications.