Abstract We developed a collagen microfiber (CMF)–based 3D cancer tissue model included monocytes. Using a sedimentation culture method, normal human dermal fibroblasts, endothelial cells, cancer cells, and THP-1 cells, that is human monocytes cell line, were co-cultured under high collagen density. THP-1 cells differentiated into macrophages and showed M2-like polarization in the 3D tumor microenvironment. This model was further applicable to drug evaluation by demonstrating pH-responsive cytotoxicity of poly(vinyl alcohol)–ursodeoxycholic acid15.
Moroishi et al. (Tue,) studied this question.