BPS2026 – Unlocking cryo-EM structures: The EMSuite server for high-accuracy modeling and analysis

Cryo-electron microscopy (cryo-EM) is a recent experimental method for determining molecular structures, providing critical insights into their functions and mechanisms. However, accurate interpretation of cryo-EM maps requires sophisticated computational tools to model precise atomic structures. The EMSuite Server, available at https://em.kiharalab.org, incorporates advanced algorithms developed at the Kihara Lab, providing researchers with an efficient and user-friendly solution for cryo-EM structure modeling, validation, and refinement. Demonstrating its significant impact and widespread adoption, the EMSuite server has processed over 6,700 jobs from more than 1,700 structural biologists worldwide, making significant contributions to diverse biological research. This presentation highlights the server’s key functionalities and well-established tools such as DAQ-Score, DeepMainmast, and CryoREAD. It demonstrates how the server’s algorithms can be effectively utilized to generate accurate structural models across a range of resolutions and structure types with multiple examples. By leveraging these tools, researchers can generate high-quality structural models that enhance the understanding of molecular functions and mechanisms.