• A sensitive SLEM platform of THY was fabricated to offer superior analytical performances • ZIF-8 MOFs decorated with gold nanoparticles was designed and prepared as amplifier • The detection limit of IL-6 is low to 0.2 ng/mL • Validated in serum with >90.3 % recovery As a dimeric protein, thyroid globulin (THY) serves as a critical biomarker for various thyroid cancers, making the development of effective detection methods highly significant. In this study, we constructed a highly sensitive sandwich-like electrochemical immunosensing platform (SLEM) for THY detection by synthesizing two key nanocomponents: ZIF-8 metal-organic frameworks (MOFs) decorated with gold nanoparticles (Au NPs) to anchor ferrocene (Fc) probes and THY secondary antibodies (Ab 2 ), and few-walled carbon nanotubes@β-cyclodextrin (FCNTs@β-CD) nanohybrids to immobilize THY primary antibodies (Ab 1 ). The unique structure of ZIF-8 MOFs enables abundant Au NP decoration, which in turn facilitates Fc and Ab2 immobilization as signal amplifiers. Concurrently, FCNTs@β-CD nanohybrids act as electrode surface carriers, combining the large surface area and conductivity of FCNTs with the superior solubility and host-guest recognition capability of β-CD for Ab 1 binding. After optimizing relevant sensing parameters, the proposed SLEM achieved excellent performance, including a wide linearity (0.5–150 ng/mL) and a low detection limit (0.2 ng/mL), suggesting its potential for practical THY detection applications.
He et al. (Sun,) studied this question.
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