Lipophilic anilino squaraine dye as a novel superluminescent probe with efficient two photon transition and high photostability

Key Points

• The aim is to develop a new squaraine dye that performs well in two-photon fluorescent microscopy.
• Synthesis of a novel squaraine structure with large lipophilic fragments.
• Incorporation of multiple hydroxy groups in the molecular core.
• Characterization for two-photon fluorescent microscopy performance.
• Demonstrated efficient near-infrared fluorescence.
• Exhibited high photostability compared to traditional fluorescent probes.
• Enabled effective imaging in microscopic applications.