Negative selection assisted isolation of a highly selective DNA aptamer for the detection of 17α-ethinylestradiol

Key Points

• This research aims to develop a highly selective DNA aptamer to detect 17α-ethinylestradiol, an environmentally concerning compound.
• Utilized negative selection techniques to isolate DNA aptamers
• Conducted binding affinity assays to measure selectivity
• Analyzed interaction with estrogenic compounds
• Successfully identified an aptamer with high selectivity for 17α-ethinylestradiol
• Demonstrated low cross-reactivity with other estrogens
• Showed effective detection in environmental samples