Design, synthesis, in vitro evaluation and in silico studies of new pyrimido4′,5′:4,5thieno2,3-dpyrimidine derivatives as potential antimicrobial, antioxidant and α-amylase inhibitors

Key Points

• New pyrimido derivatives show significant antimicrobial and antioxidant activities, demonstrating potential therapeutic value.
• The most potent derivatives exhibit over 70% efficacy in inhibiting microbial growth under specific conditions.
• Assessment through in silico studies suggests favorable binding interactions with α-amylase, supporting further investigation.
• These findings highlight the need for advanced studies to confirm these in vitro results in clinical settings.