FastqOrienter: A Python utility for the automated orientation and quality diagnostics of paired-end Illumina metabarcoding reads

Key Points

• Automated processing allows for improved accuracy in quality diagnostics of paired-end reads, streamlining analysis.
• Key improvements include a 20% increase in processing speed and a significant reduction in manual errors.
• Analysis utilized bioinformatics techniques to assess the quality and orientation of Illumina-generated sequences across multiple datasets.
• Enhancing pipeline efficiency, this tool supports researchers in managing complex metabarcoding projects for biodiversity assessments.