CRISPR/Cas12a and fork-shaped probe enhance LAMP–LFT integration for equipment-free detection of Listeria monocytogenes

Key Points

• Detection of Listeria monocytogenes improved significantly using a combined approach.
• The method integrates CRISPR/Cas12a with LAMP and LFT for rapid results.
• A fork-shaped probe enhances the specificity and sensitivity of the detection process.
• This finding supports developing accessible and efficient testing methods for foodborne pathogens.