Développement d'une méthode LC-MS/MS pour la quantification du 7-alpha-hydroxy-4-cholesten-3-one (C4) dans le sérum humain

Key Points

• The method allows for accurate quantification of 7-alpha-hydroxy-4-cholesten-3-one, a significant biomarker for cholesterol metabolism.
• A sensitivity of 0.1 ng/mL was achieved during the quantification process, ensuring precise measurements across samples.
• The procedure employs lc-ms/ms, which enhances detection capabilities for complex biological samples.
• Potential implications include improved monitoring of cholesterol levels and related metabolic conditions.