March 3, 2026Open Access

Uspešno čiščenje DNK iz agaroznih čepkov PFGE za sekvenciranje celotnega genoma

Key Points

Bacterial whole-genome sequencing can be effectively performed on DNA from agarose plugs, enhancing methods for genotyping.
The study confirms the suitability of genomic DNA extracted from long-stored PFGE agarose plugs for WGS.
Analysis of PFGE agarose plugs reveals a viable alternative for genotyping when bacterial isolates are inaccessible.
Findings support the use of stored agarose plugs for future genetic studies and applications.

Abstract

Whole-genome sequencing (WGS) has replaced Pulsed-field gel electrophoresis (PFGE)-based bacterial genotyping as the reference genotyping method. We investigated the suitability of purified genomic DNA extracted from PFGE agarose plugs stored in a laboratory collection for WGS in cases where bacterial isolates are no longer available. Our study has shown that bacterial WGS can be successfully performed on DNA extracted from PFGE agarose plugs.

Uspešno čiščenje DNK iz agaroznih čepkov PFGE za sekvenciranje celotnega genoma

Key Points

Abstract

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